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DNA82034
EZ DNA Capture for Real Time PCR Quantitation


Product Description and Features:

Real-time PCR (qPCR) is a highly sensitive technique for quantifying DNA, which finds extensive applications in DNA bioanalysis for cell and gene (mRNA, viral and vaccine) therapies.

Often sample matrix interferes with qPCR DNA quantitation and must be removed through a DNA sample purification, such as sodium iodide-based purification (Ivy Fine Chemical catalog B48202), Qiagen column or filter plate, magnetic bead etc. All these methods require multiple steps (including washing) and good skills from analysts. Assay variability and DNA loss are common. Instrument investment and long hours in executing experimental steps are a burden to the industry and academic labs.

Here comes an innovative, quick, and easy method: 1) DNA samples are simply incubated with a uniquely formulated buffer containing Proteinase K and specific biotinylated primers of your choice. Protein interference will be reduced, and biotin primers will bind to target DNA. 2) Transfer incubated samples to our Streptavidin-coated Capture PCR plate. DNA target will bind to the Capture PCR plate and remaining interference matrix (including salts and residual proteins) is removed by pipetting out, plate washer or plate flipping on paper towels. Additional one washing may not be needed pending upon the complexity of your sample matrices and needs to be tested during your method development. 3) Real time PCR is performed directly on SAME Capture PCR plate without a need of a DNA elution and transfer to eliminate DNA loss and to reduce assay variability.

Our assay kit is simple and quick. You don’t need any special lab skills and don’t need to invest in any instruments except real time PCR. Our solution-based assay procedures can be automated at a liquid handler.
  

Catalog # Name Package Size Price
DNA82034 EZ DNA Capture for Real Time PCR Quantitation 96 DNA Capture and PCR Reactions $760.00

The manual is available in pdf format.

Notes:

  • Stable at 4 oC for 12 months.
  • Laboratory Use Only.
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